The Science Underlying ImmunO4


Selected Founder's Publications

 

  1. Hewitt CW, Sawyer S, Beck PA, Martin DC: A simple method for the isolation of platelet-free lymphocyte suspensions from rat whole blood. J Immunol Methods. 36: 227-234, 1980.
  2. Hewitt CW, Moskowitz D, Osborne JG, Nelson MJ, Martin DC: The production of large quantities of ascitic fluid for kidney allograft enhancement in rats. Transplantation. 33: 228-232, 1982.
  3. Hewitt CW, Black KS, Dowdy SF, Gonzalez GA, Achauer BM, Martin DC, Furnas DW, Howard EB: Composite tissue (limb) allografts in rats: III. Development of donor-host lymphoid chimeras in long-term survivors. Transplantation, 41: 39-43, 1986.
  4. Hewitt CW, Black KS, Quintero CS, Slater KJ, Martin DC: Two-dimensional immunoelectrophoretic analysis of urinary ultraconcentrates: Antigenic differences between bladder cancer positive patients, normal individuals, and patients with urinary tract infections. J Surg Oncol, 36(3): 193-197, 1987.
  5. Hewitt CW, Black KS, Gonzalez GA, Dowdy SF, Achauer BM, Howard EB: Decreased reactivity of allosera against target lymphocytes obtained following thermal injury or long-term cyclosporine treatment. Clin Immunol Immunopathol, 45(3): 395-404, 1987.
  6. Hewitt CW, Black KS, Gonzalez GA, Dowdy SF, Reyes M Jr, Martin DC, Achauer BM: Long-term residual cyclosporine levels following short-term administration in various allograft models demonstrating extensive survival prolongation. Transplant Proc, 19: 1244-1245, 1987.
  7. Hewitt CW, Black KS, Henson LE, Achauer BM, Nguyen JHT: Lymphocyte chimerism in a full allogeneic composite tissue (rat-limb) allograft model prolonged with cyclosporine. Transplant Proc, 20(2): 272-278, 1988.
  8. Hewitt CW, Black KS, Aguinaldo AA, Lin SS, Howard EB: Pathologic alterations in the skin component of composite tissue and skin allografts treated with cyclosporine. Transplant Proc, 20(3): 1003-1004, 1988.
  9. Ulich TR, Rong-Xiang N, Hewitt CW, Black KS, Go C, Martin DC: The development of humoral immunity to tissue-specific tubular basement membrane alloantigens after renal transplantation across the major histocompatibility barrier in rats immunomodulated with blood transfusions and cyclosporine. Proc Soc Exp Biol Med, 188: 328-334, 1988.
  10. Henson LE, Hewitt CW, Black KS: Use of regression analysis and the complement-dependent cytotoxicity typing assay for predicting lymphoid chimerism. J Immunol Methods, 114: 139-144, 1988.
  11. Hewitt CW, Black KS, Henson LE, Dowdy SF, Philosophe B, Martin DC: Serologic properties of the triple antibody-sandwich-lymphocyte-agglutination assay (TASLA). J Clin Lab Immunol, 28: 45-50, 1989.
  12. Caiozzo VJ, Black KS, Hewitt CW, Grisham GR, Achauer BM, Prietto CA: Histochemical properties of muscle allografts enhanced via cyclosporine. Transplantation, 48: 840-844, 1989.
  13. Walker KW, Llull R, Balkian GK, Ko HS, Flores KM, Black KS, Hewitt CW, Martin DC: A rapid and sensitive cellular enzyme linked immunoabsorbent assay (CELISA) for the detection and quantitation of antibodies against cell surface determinants: I. A comparison of cell fixation and storage techniques. J Immunol Methods, 154: 121-130, 1992.
  14. Woolley DS, Hou A, Strande LF, Tatem LD, Matthews M, Hewitt CW: Vascularized bone marrow transplantation and graft versus host disease: Morphometric analysis of muscle. Transplant Proc, 26(6): 3321-3322, 1994.
  15. Puglisi RN, Doolin EJ, Hewitt CW: Morphometric analysis of rat renal allograft tolerance by computerized digital image processing. Transplant Proc, 27(1): 555-556, 1995.
  16. Tatem LD, Hou A, Black KS, Llull R, Hewitt CW: Digital image analysis of MHC Class I and Class II expression during site-specific immune suppression with topical cyclosporine. Transplant Proc, 27(1): 344-345, 1995.
  17. Hewitt CW, Englesbe MJ, Tatem LD, Strande LF, Doolin EJ, Dalsey RM, DeLong WG: Graft versus host disease in extremity transplantation: Digital image analysis of bone marrow In Situ. Ann Plast Surg, 35(1): 108-112, 1995.
  18. Salartash K, Hewitt C, Lee L, Foley S, Born C, DeLong W: High pressure irrigation destroys cellular architecture and causes progressive necrosis of skeletal muscle and high levels of tumor necrosis factor. Surg Forum, 66: 595-597, 1995.
  19. Balkian GK, Flores KM, Llull R, Ko HS, Walker KW, Black KS, Hewitt CW: A rapid and sensitive cellular enzyme-linked immunoabsorbent assay (CELISA) for the detection and quantitation of antibodies against cell surface determinants. II. Optimal reagent concentrations and predictive analysis. Cell Transplant, 6(4): 431-437, 1996.
  20. Hewitt CW, Woerner T: Histologic evaluation of various conventionally and immunostained specimens by novel high definition 3-D microscopy with multiple oblique illumination. The FASEB Journal, Vol. 10(6), p. A1290, 1996.
  21. Ramsamooj R, Llull R, Tatem LD, Black KS, Lotano V, Dalsey RM, Born CT, DeLong WG, Hewitt CW: Graft versus host disease in limb transplantation: Digital image analysis of bone marrow and TGF-ß Expression In Situ Using a Novel 3-D Microscope. Transplant Proc, 28(4): 2029-2031, 1996.
  22. Ramsamooj R, Patel MP, Llull R, Levin SI, Black KS, Hewitt CW: Use of regression analysis and flow cytometry for determining levels of mixed semiallogeneic immune chimerism. J Invest Surg, 9: 273-281, 1996.
  23. Tatem LD, Hirpara S, Dalsey RM, Iannacone WM, DeLong WG, Hewitt CW: The role of in situ IL-2r and TGF- expression in tolerant vascularized bone marrow (limb) transplant (VBMT) chimeras. Transplant Proc, 29(4): 2194-2195, 1997.
  24. Hewitt CW, Strande L, Santos M, Goodman M, Tarnoff M, Zaontz MR, DelRossi AJ, Doolin EJ: Rat renal allograft tolerance is associated with local TGF- and absence of IL-2r expression within chimeric immunocytic foci. Transplant Proc, 29(4): 2183-2184, 1997.
  25. Goodman MD, Tarnoff ME, Strande LF, Tatem L, Kain MS, Doolin EJ, DelRossi AJ, Hewitt CW. The relationship between in situ chimerism, local TGF-B and IL-2r expression in rat allograft tolerance. Surg Forum 48: 508-509, 1997.
  26. Woerner T, Hewitt CW. (Presentation) Stability and Sensitivity of a Novel Tetramethyl Benzidine (TMB) Formulation for Immunoassay. Proceedings of the Ninety-Ninth General Meeting of the American Society for Microbiology, Chicago, Illinois, May 30-June 3, 1999.
  27. Hewitt CW, Doolin E, Tran H, Woerner T. Sensitivity of various commercial tetramethyl benzidine (TMB) formulations for immunoassay. The FASEB Journal, 13(5): 1999.
  28. Woerner T, Hewitt CW. Stability and Sensitivity of a Novel Tetramethyl Benzidine (TMB) Formulation for Immunoassay. Proceedings of the Ninety-Ninth General Meeting of the American Society for Microbiology, p. 661, 1999.
  29. Hewitt C and Woerner T. A unique tetramethyl benzidine (TMB) formulation for immuno- and molecular assay. Proceedings of the 51st Annual Meeting and Clinical Laboratory Exposition of the AACC, p. 39, 1999.
  30. Hewitt CW, Tarnoff M, Goodman M, Ramsamooj R, Chin V, Doolin EJ: Use of 3-D microscopy with multiple oblique illumination in evaluating cytopathology preparations. Anal Quant Cytol Histol, 21(2): 139-142, 1999.
  31. Doolin EJ, Strande LF, Chen MK, Kain MS, Hewitt CW. The effect of leukocyte infiltration on apoptosis in an in vitro thermal injury bioartificial living skin equivalent model. J Burn Care Rehabil, 20(5): 374-376, 1999.
  32. Doolin EJ, Geldziler B, Strande L, Kain M, Hewitt CW. The effects of microgravity on growing cultured skin constructs. Tissue Engineering, 5(6): 573-581, 1999.
  33. Puc MM, Chrzanowski FA, Tran H, Geldziler B, Martucci M, Doolin EJ, Hewitt CW. Multiple oblique illumination and high definition microscopy for breast fine needle aspirates. J Invest Surg, 13: 259-263, 2000.
  34. Hewitt CW, Tran JLV, Doolin EJ, Woerner T. The effect of pH on diaminobenzidine (DAB) tissue staining by antibody conjugated horseradish peroxidase (HRP) catalysis. Proceedings of the 100th General Meeting of the American Society for Microbiology, p. 58, 2000.
  35. Hewitt CW, Marra SW, Kann BR, Tran HS, Puc MM, Chrzanowski FA, Tran JLV, Lenz SD, Cilley JH, Simonetti VA, DelRossi AJ. BioGlue surgical adhesive for thoracic aortic repair during coagulopathy: Efficacy and histopathology. Ann Thorac Surg, 71(5), 1609-1612, 2001.
  36. Albaugh G, Kann G, Strande L, Vemulapalli P, Hewitt C, Alexander JB. Nicotine induces endothelial TNF- expression, which mediates growth retardation in vitro. J Surg Res, 99: 381-384, 2001.
  37. Elkins RC, Goldstein S, Hewitt CW, Walsh SP, Dawson PE, Ollerenshaw JD, Black KS, Clarke DR, O’Brien MF. Recellularization of heart valve grafts by a process of adaptive remodeling. Semin Thorac Cardiovasc Surg, 13(4Suppl1): 87-92, 2001.
  38. Ramsamooj R, Doolin E, Greenberg G, Catalano E, Hewitt CW. Real time high definition 3-dimensional microscopy for evaluating problematic cervical pap smears classified as atypical squamous cells of undetermined significance (ASCUS). Cancer, 96(3): 181-186, 2002.
  39. Van Tran JL, Reid L, Armour R, Tai C, Eydelman R, Woerner T, Rosebrock J, Woerner H, Hewitt C. Enhanced detection of immunoperoxidase in Western Blots utilizing H2O2 under alkaline conditions with stannous fluoride. Proceedings of the 102nd General Meeting of the American Society for Microbiology, p. 491, 2002.
  40. Rosebrock JA, Woerner T, Woerner HL, Hewitt C. Stabilization of H202 under Alkaline Conditions for use in Luminescence Assays for enhanced detection of peroxidase. Proceedings of the 43rd Interscience Conference on Antimicrobial Agents and Chemotherapy Annual Meeting, Chicago, Illinois, September 14-17, 2003.
  41. Woerner T, Tran JLV, Bellavance E, Hadeed J, Kumar S, Marra S, Hewitt CW. An alkaline phosphatase red immuno-detection system with both chromophore and fluorophore properties. The FASEB Journal, 18(5): A784, 2004.
  42. Albaugh G, Bellavance E, Strande L, Heimburger S, Hewitt CW, Alexander JB. Nicotine induces mononuclear leukocyte adhesion and expression of adhesion molecules, VCAM and ICAM, in endothelial cells in vitro. Ann Vasc Surg, 18(3): 302-307, 2004.
  43. U.S. Patent 7,001,717; Application 10/727,537. Charcoal stabilization of phenyl phosphates. Inventor: Woerner TM. Patent date: February 21, 2006.
  44. Aghai AH, Kumar S, Farhath S, Kumar M, Saslow J, Nakhla T, Eydelman R, Strande L, Stahl G, Hewitt C, Nesin M, Rahman I. Dexamethasone Suppresses Expression of Nuclear Factor-kappaB in the cells of tracheobronchial lavage fluid in premature neonates with respiratory distress. Pediatr Res., 59(6): 811-815, 2006. Jun
  45. Martens M, Kumar MM, Kumar S, Goldenberg M, Kawata M, Pennycooke O, Strande L, Hadeed J, Camacho J, Hewitt C, Slotman GS. Quantitative analysis of organ tissue damage after septic shock. American Surgeon, 73(3): 243-248, March 2007.
  46. U.S. Patent 7,214,481; Application 11/262,715. Charcoal stabilization of phenyl phosphates. Inventor: Woerner TM. Patent date: May 8, 2007.
  47. Lefebvre DR, Strande LF, Hewitt CW. An enyzme-mediated assay to quantify inoculation volume delivered by suture needlestick injury: Two gloves are better than one. J Am Coll Surg, 206(1): 113-122, January 2008.
  48. Cendales LC, Kanitakis J, Schneeberger S, Burns C, Ruiz P, Landin L, Remmelink M, Hewitt CW, Landgren T, Lyons B, Drachenberg CB, Solez K, Kirk AD, Kleiner DE, Racusen L.The Banff 2007 Working Classification of Skin-Containing Composite Tissue Allograft Pathology. Am J Transplant, 2008 Apr 29.
  49. U.S. Patent 7,381,797; Application 10/141,177. Stablization of H2O2 Under Alkaline Conditions for Use in Luminescence, Fluorescence and Colorimetric Assays for Enhanced Detection of Peroxidase Type Assays. Inventors: Woerner TM, Rosebrock JA, Hewitt CW, Woerner HL. Patent date: June 3, 2008.
  50. U.S. Patent 7,563,566; Application 60289517. Stablization of H2O2 Under Alkaline Conditions for Use in Luminescence, Fluorescence and Colorimetric Assays for Enhanced Detection of Peroxidase Type Assays. Inventors: Woerner TM, Rosebrock JA, Hewitt CW, Woerner HL. Patent date: July 21, 2009.